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Apex Hot Start Master Mix, 2X
產品型號:Cat #: 42-197
簡介:


1.5mM MgCl2 (Final)
100 Reactions/Unit
Brand: Apex Bioresearch Products
Apex Hot Start Master Mix, 1.5mM MgCl2 (Final Conc.) with Ammonium Buffer that promotes robust amplification, h

Apex Hot Start Master Mix, 2X  的詳細介紹

Apex Hot Start Master Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications. Apex Hot Start DNA Polymerase is a modified form of Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Apex Hot Start DNA Polymerase, ammonium buffer system, dNTPs and magnesium chloride are present in Apex Hot Start Master Mix with Apex Buffer I. Each reaction requires 25ul of the 2.0X reaction mix.

For most standard applications Master Mix based on Ammonium Buffer I is the best option. It promotes robust amplification, high yield and high specificity. In certain cases Master Mix with Buffer II is the best option.

Product Overhang 3'-dA
Hot-Start Yes
5' - 3' Exonuclease activity Yes
Concentration 2X
Loading Dye No
Storage Long term storage (3 years) at -20°C Short term storage (6 months) at 4°C
Max Amplicon Length 5kb
Polymerase inhibitor Chemical
Composition The expiration date is labeled on each individual tube and is dependent on proper long term storage conditions
Expiration Product expiration date at -20 °C is stated on the tube label.
Packaging Configuration 2 x 1.25ml


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