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qPCRBIO HRM Mix
產(chǎn)品型號(hào):Cat #: 17-520B
簡(jiǎn)介:


5 x 1ml HRM Master Mix
500 x 20ul Rxns/Unit
Brand: PCR Biosystems
Accurate and fast detection of genetic mutations and SNPs
Detects subtle sequence variations including class I to

qPCRBIO HRM Mix  的詳細(xì)介紹

High Resolution Melt (HRM) analysis is a powerful post-PCR analysis method used to detect and identify mutations, polymorphisms and epigenetic differences in double-stranded DNA samples.

qPCRBIO HRM Mix uses the novel SyGreen 2 intercalating dye. SyGreen 2 does not inhibit PCR and is a saturating, 3rd generation dye essential for accurate SNP genotyping. Unlike first generation dyes, SyGreen 2 can be used at high concentrations and does not have a different affinity for AT or GC rich sequences. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer market leading performance with minimal or no optimisation. qPCRBIO HRM Mix delivers accurate detection of subtle sequence variations including class I to class IV mutations and CpG methylation differences. Many modern real-time PCR instruments have a fast cycling mode. The same developments that improve the sensitivity and consistency of qPCRBIO HRM Mix in standard cycling conditions also allow for industry leading performance in fast and ultra fast cycling conditions.

Reactions 500 x 20ul Rxns/Unit
Packaging 5 x 1ml HRM Master Mix
Storage Between -30 and -15°C
MgCl2 Concentration Mix contains 12mM for final reaction concentration of 3mM
Compare To Applied Biosystems™ MeltDoctor™ HRM Master Mix, Qiagen Type-it HRM PCR Kit, KAPA HRM FAST qPCR Kit Master Mix 2x


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