The clone 4G7 recognizes a 90-kDa CD19 antigen that is present on human B lymphocytes. CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. The CD19 antigen is present on approximately 7% to 23% of human peripheral blood lymphocytes and on spleenocytes, and on human B lymphocytes at all stages of maturation but is lost on terminally differentiated plasma cells. CD19 does not react with resting or activated T lymphocytes, granulocytes, or monocytes.
Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5μg (unconjugated, Biotin) or 5μl (conjugated) of these products are sufficient for staining 1 million cells in a 100μl staining volume or 100μl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at [email protected].