The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 μg (per prep) of high-quality RNA directly from samples in TRI-Reagent? or similar*. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product. The procedure is easy: simply apply a sample in TRI-Reagent? to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs ( ≥ 17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 5-10 minutes.
The RNA binding capacity of the supplied Zymo-Spin™ IIC Column is ~50 μg with a minimal elution volume of 25 μl.