The ZymoBIOMICS? DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS? lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads™.This kit is equipped with our OneStep™ PCR Inhibitor removal technology, enabling PCR from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Sample Sources: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from mammalian feces, soil, fungal/bacterial cells, biofilms and water. Elution Volume: = 37.5 μl DNA Elution Buffer or DNase free water. Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield™. Equipment: Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block. Sample Storage: Eluted DNA should be stored at = -20oC.. Purity: High quality, inhibitor-free DNA is eluted with ZymoBIOMICS™ DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing. Yield: Up to 10 μg total DNA can be eluted into 50 μl (37.5 μl minimum). Processing Volume: Fecal =100 mg, Soil = 200 mg, Cells = 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells). Type: Total DNA. Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing.